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21. Architecture-inspired paradigm for 3D bioprinting of vessel-like structures using extrudable carboxylated agarose hydrogels

September 2019

Aurelien Forget*, Tiziano Derme, Daniela Mitterberger, Markus Heiny, Caleb Sweeney, Likhith Mudili, Tim R Dargaville, V Prasad Shastri

3D bioprinting as a process has enabled the creation of complex structures laden with biological information including cells that is not realizable with conventional manufacturing techniques. Within this new manufacturing process, design strategies and design tools have to be adapted to deliver its full potential. In architecture, parametric design has allowed the creation of new shapes by expressing parameters in an algorithm that defines the relationship between design intent and design response. In this study, the application of this method to the bioprinting of hollow channel grown in silico within a porous scaffold is demonstrated. The final object was printed using a novel bioink formulation based on carboxylated agarose that is extrudable at ambient temperature. The hollow bioprinted object was imaged with X-ray computed tomography to obtain a 3D model for design validation. This proposed workflow “from design to validation” represents a new paradigm for realizing complex structures in 3D bioprinting through in silico optimization.